Confocal Raman Microscopy by Thomas Dieing, Olaf Hollricher, Jan Toporski


By Thomas Dieing, Olaf Hollricher, Jan Toporski

Confocal Raman Microscopy is a comparatively new procedure that enables chemical imaging with no particular pattern training. through integrating a delicate Raman spectrometer inside of a cutting-edge microscope, Raman microscopy with a spatial solution right down to 200nm laterally and 500nm vertically could be accomplished utilizing obvious gentle excitation. contemporary advancements in detector and machine expertise in addition to optimized software layout have diminished integration instances of Raman spectra through orders of importance, in order that whole pictures such as tens of hundreds of thousands of Raman spectra will be obtained in seconds or mins instead of hours, which was average only one decade in the past. the aim of this ebook is to supply the reader a entire evaluate of the quickly constructing box of Confocal Raman Microscopy and its applications.

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The choice of the pinhole size is very important in Raman experiments. On one hand the signal should be as high as possible, while on the other hand the image should be confocal. 5 to avoid a loss in z-resolution. 5. In practice, the pinhole size can be up to v Pmax = 4 without significantly changing depth resolution and up to v Pmax = 2 without significantly changing lateral resolution. As shown in Sect. 1, for v Pmax > 4, only the resolution of a conventional microscope remains. For the experiment, the relation π d0 M ≥ NA v Pmax λ must be fulfilled, where M is the magnification, d0 the diameter of the pinhole, and N A the numerical aperture of the objective.

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