Chemical and Biochemical Applications by Pierre Laszlo (Eds.)

Chemical Engineering

By Pierre Laszlo (Eds.)

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This approach w a s u s e d by F r e e m a n and a s s o c i a t e s for the detection of 13 C couplings in natural abundance ( B a x et al, 1980a,b). 1%, only one site in 10 is C and has one 13 1 3 C neighbor. The experiment relies on observing only the signals from C 1 3 sites that have C neighbors. The selectivity arises from detecting only signals that once existed a s c a r b o n - c a r b o n double-quantum coherence. This experiment, known a s I N A D E Q U A T E , gives a one-dimensional 13 1 3 spectrum which is the C - c o u p l e d C spectrum.

At present there are only a few examples of this potentially useful approach. The utility of the multi­ ple-quantum experiment is that, although both chemical shifts and split­ tings due to s p i n - s p i n couplings scale with the number of quanta in­ volved, the line widths can remain constant, or even d e c r e a s e , with the number of quanta. This approach is especially appealing in applications to quadrupolar nuclei which tend to have rather broad lines. The multiple2 1 4 quantum technique has been applied to H and N to obtain high-resolu­ tion spectra (Prestegard and Miner, 1981).

C 1 3 C 1 3 d e c o u p l i n g is u s e d in p u l s e s e q u e n c e ( d ) t o s u p p r e s s t h e cross-hatched areas indicate decoupling. C coupling o f the protons. T h e 2. T e c h n i q u e s T h a t E n h a n c e S e n s i t i v i t y a n d I m p r o v e R e s o l u t i o n 41 180° out of p h a s e with one another and to permit efficient magnetization transfer which is solely dependent on the I spin decoupled S chemical shift. The magnetization transfer is completed by the I pulse at the end of t\ + Δ Η .

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