Cell Culture Engineering IV: Improvements of Human Health by Barry C. Buckland (auth.), Barry C. Buckland, John G.


By Barry C. Buckland (auth.), Barry C. Buckland, John G. Aunins, Theodora A. Bibila, Wei-Shou Hu, David K. Robinson, Weichang Zhou (eds.)

Cell tradition Engineering IV, advancements of Human Health covers the most recent techniques to enhancing the phone host via stronger knowing of the molecular biology, the improvement of novel vaccines, ways to bioreactor layout and operation, tracking strategies in strategy keep an eye on and caliber comparable subject matters. The paintings used to be conscientiously prepare as one results of the cellphone tradition Engineering IV assembly held in San Diego, U.S.A. in 1994, notwithstanding, the publication is probably not perceived as a lawsuits quantity - the factors of the ebook sequence follow.
For telephone biologists, biochemists, molecular biologists, immunologists and different disciplines regarding cellphone tradition engineering, operating within the educational setting, in addition to in (biotechnology or pharmaceutical) industry.

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The apparent concIusion is that the cancer cellS' inability to produce highly unsaturated EFAs is connected to their increased susceptibility to EFA-mediated growth inhibition or killing. In addition, it is interesting to note that desaturation ofFAs and detoxification of oxidation products both require NAD-dependent enzymes, but the relevance of this cofactor remains to be determined. A secondary consequence of the lack of 6-desaturated EFAs may be an increase in the levels of antioxidants. In fact, many malignant cells do exhibit increased levels of antioxidants (Horrobin, 1990).

We conclude by presenting the growth effects of a variety of EFAs on two mammary epithelial cell Iines, one neoplastic and the other non-cancerous, along with an analysis of the likely mechanisms. Membrane-associated effects The EFA composition of cells in vivo reflects the prevailing EFA family in the diet of the organism. Supply of specific FAs or FA mixtures to cells in culture invariably leads to changes in the cellular FA composition that reflect the exogenous additions (Spector and Yorek, 1985).

In conventional batch culture, the antibody concentration increased very slowly during the first 50 hours due to the low cell density and reached only 50 mg I-I when the cells beg an to die. In the fed-batch experiment, antibody concentration increased nearly exponentially at the beginning due to the higher cell density and viability. Unlike the batch experiment, the antibody concentration increased continuously until the end of the culture to 900 mg I-I. Table 11 shows the comparison of culture performances among batch and fed-batch (T-ftask and 2-liter bioreactor) experiments.

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