Bioengineering in Cell and Tissue Research by Stefan Golz (auth.), Gerhard M. Artmann, Shu Chien (eds.)

Technique

By Stefan Golz (auth.), Gerhard M. Artmann, Shu Chien (eds.)

This ebook studies the most recent technological advancements of bioengineering ways in cellphone and tissue examine. it's intended to have lifestyles and spirit, and to turn into a pioneer in expertise and sciences, specifically the lifestyles technology. The chapters are written by means of first-class scientists on complex, frontier expertise and deal with medical questions that desire significant considering by way of engineering. The goals are to supply the readers, together with scholars, college, and all scientists operating in academia and undefined, new info on bioengineering in mobilephone and tissue examine to augment their figuring out and innovation.

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The attractiveness of secreted luciferases as reporters is a strong stimulus for the investigation and exploitation of new bioluminescent systems. Metridia longa is a small luminous marine copepod (Fig. 3D). The bioluminescence originates as a secretion from epidermal glands located in the head part and abdomen in response to mechanical, electrical, or chemical stimuli. Bioluminescence in Metridia longa may well serve as a defense mechanism against predators; the release of a luminous bolus from the animal is accompanied by rapid swimming that displaces the copepod away from its “glowing phantom”.

This luciferase emits light at a peak of 480 nm with a broad emission spectrum extending to 600 nm. Gaussia luciferase has been cloned, overexpressed in bacteria, and used as a sensitive analytical reporter for hybridization assays and monitoring of cellular expression in culture and in vivo (Tannous et al. 2005). 7 Coelenterazine Dependent Luciferases 9 Fig. 3 Origins of Glow-light reporter genes used in uHTS. Picture of A Firefly Beetle (identified luciferase: Firefly luciferase), B Gaussia princeps (identified luciferase: Gaussia luciferase; GLuc), C Renilla reniformis (identified luciferase: Renilla luciferase) and D Metridia longa (identified luciferase: Metridia luciferase; MLuc) ciferase is a coelenterazine dependent luciferase which does not require ATP for activity.

Dlctyostellum, plants, Drosophila and mammalian cells). In the last 10 years green fluorescent protein (GFP) has changed from a nearly unknown protein to a commonly used tool in molecular biology, medicine, and cell biology. GFP is used as a biological marker and reporter gene. Green fluorescent proteins are found in numerous organisms such as jellyfish and sea pansies. In 1955 it was first reported that Aequorea (Aequorea victoria) fluoresced green when irradiated with ultraviolet light. Two proteins in Aequorea are involved in its bioluminescence, aequorin (a flash-light type coelenterazine-dependent photoprotein) and the green fluorescent protein (GFP).

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